The microdissection technique described to isolate the ICM from the blastocyst can be substituted with an immunosurgery technique modified from a procedure developed for derivation of mouse ESCs. Briefly, after hatching, the blastocysts are incubated with an anti-human cell surface antibody that binds to the external layer, the trophoblast. After rinsing away excess antibody the embryos are treated with guinea-pig complement. The complement-antibody binding initiates lysis of the trophoblast. The ICM is protected because it was not accessible to the antibody. For further information see Mitalipova et al. (2003).

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