The microdissection technique described to isolate the ICM from the blastocyst can be substituted with an immunosurgery technique modified from a procedure developed for derivation of mouse ESCs. Briefly, after hatching, the blastocysts are incubated with an anti-human cell surface antibody that binds to the external layer, the trophoblast. After rinsing away excess antibody the embryos are treated with guinea-pig complement. The complement-antibody binding initiates lysis of the trophoblast. The ICM is protected because it was not accessible to the antibody. For further information see Mitalipova et al. (2003).
Was this article helpful?