Visualization of Exosomes Using Electron Microscopy

Exosomes (10 |L) purified by differential ultracentrifugation and resuspended in PBS are loaded on a Formvar/carbon coated grid for 1 min, negatively stained with 10 |L neutral 1% aqueous phosphotungstic acid or 1% uranylacetate for 1 min and viewed using a JEM-1011 computer controlled high contrast 80 kV transmission electron microscope. Exosomes are typically 50-120 nm in diameter and appear "saucer" shaped. Figure 2 shows an electron micrograph of exosomes secreted from unmodified DC.

Exosome Under Microscope
Resuspend exosome pellet in PBS plus/minus protease inhibitors Fig. 1. Exosome purification scheme.

Was this article helpful?

0 0
10 Ways To Fight Off Cancer

10 Ways To Fight Off Cancer

Learning About 10 Ways Fight Off Cancer Can Have Amazing Benefits For Your Life The Best Tips On How To Keep This Killer At Bay Discovering that you or a loved one has cancer can be utterly terrifying. All the same, once you comprehend the causes of cancer and learn how to reverse those causes, you or your loved one may have more than a fighting chance of beating out cancer.

Get My Free Ebook


Post a comment