Visualization of Exosomes Using Electron Microscopy

Exosomes (10 |L) purified by differential ultracentrifugation and resuspended in PBS are loaded on a Formvar/carbon coated grid for 1 min, negatively stained with 10 |L neutral 1% aqueous phosphotungstic acid or 1% uranylacetate for 1 min and viewed using a JEM-1011 computer controlled high contrast 80 kV transmission electron microscope. Exosomes are typically 50-120 nm in diameter and appear "saucer" shaped. Figure 2 shows an electron micrograph of exosomes secreted from unmodified DC.

Exosome Under Microscope
Resuspend exosome pellet in PBS plus/minus protease inhibitors Fig. 1. Exosome purification scheme.

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