Chapter 7: UNRAVELING THE HUMAN GENOME AND ITS FUTURE IMPLICATIONS FOR CARDIOLOGY
; Figure 7-1 : Illustrated here is the means of detecting genes or their mutations. Oligonucleotides are single short strands of DNA of about 15 to 30 bases artificial synthesized to have the sequence of the desired gene. These oligonucleotides are bound to glass with each of the four bases labeled with a distinct fluorescent color. The DNA extracted from the patient's white blood cells is denatured into separate strands and brought in contact with the artificial oligonucleotides DNA. If the sequence in the patients DNA is complementary to the oligonucleotide, hybridization will occur and the laser beam will detect the appropriate colors. If there is a mutation present, there will be a mismatch and a different color will be exhibited by the laser indicating where the mutation is.
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