Therapeutic Drug Monitoring TDM

New methods for its control. 7. Bratton, A. C. and Marshall, E. K., Jr. (1939) A new coupling component for sulfanilamide determination. 8. Brodie, B. B. and Udenfriend, S. (1943) The estimation of Atabrine in biological fluids and tissues. 9. Smith, T. W., Butler, V. P. and Haber, E. (1969) Determination of therapeutic and toxic serum digoxin concentrations by radioimmunoassay. 10. Thompson, R. D., Nagasawa, H. T. and Jenne, J. W. (1974) Determination of...

Info

Color, a variation made necessary by the use of sodium sulfite. The uric acid reagent must invariably be added after, and not before, the addition of the sodium carbonate, because in acid solution the sulfite will itself give a blue color with the phosphotung-stic acid. It may also be worth while to mention that the peculiar increase in blue color obtained by the use of cyanide is not obtained in the presence of sulfite. Opinions will doubtless differ as to whether this is an advantage or...

Leuci Mesensitive Hypoglycemia

Hypoglycemia

Fasting plasma insulin concentrations in various groups of subjects. The subject with plasma insulin concentration greater than 1,500 xU per ml had an islet cell adenocarcinoma with widespread metastases (patient of Dr. J. Field). tion depends on the precise moment of sampling. A very rapid and pronounced fall from the peak concentration would be anticipated in the case of a single secretory spurt. Conversely, a sustained elevation or continued rise in insulin concentration implies a...

Contamination

There are many sources of contamination, in the clinical laboratory. Some of these may affect a random specimen and be overlooked or not detected, even with inclusion of standards and controls. Thorough cleaning of glassware is essential. Detergents frequently contain large quantities of sodium and inorganic phosphate, and these will interfere if not completely removed. A simple check to detect residual detergent consists of filling pipets or rinsing glassware with a dilute solution of...

Enzymelinked Immunosorbent Assay

QUANTITATIVE ASSAY OF PROTEIN ANTIGEN, IMMUNOGLOBULIN G, BY MEANS OF ENZYME-LABELLED ANTIGEN AND ANTIBODY-COATED TUBES EVA ENGVALL, KARIN JONSSON and PETER PERLMANN From the Department of Immunology, The Wenner-Gren Institute, University of Stockholm, Stockholm (Sweden) Alkaline phosphatase from calf intestinal mucosa has been conjugated to a protein antigen, rabbit IgG. Such conjugates, prepared by glutardialdehyde, have been used in a competitive solid phase immunoassay. In this test...

Immunoassay Of Endogenous Plasma Insulin In Man

Centrations observed in diabetic subjects during the glucose tolerance test are not inconsistent with the less extensive data of Bornstein and Lawrence (1) and Baird and Bornstein (39). Very recently Seltzer and Smith (41), employing the rat diaphragm assay of Vallance-Owen and Hurlock (33), have reported insulin concentrations one hour after glucose, in tolbutamide-sensitive adult diabetics, almost in the normal range, but significantly lower values were observed in juvenile diabetics and...

180 Mg 100 Ml Of Blood Sugar

It is evident that variation in the volumes of solution applied to the paper strips is of no consequence. Generally 100 to 200 fil is applied, the larger volume permitting use of a smaller quantity of tracer beef insulin-I131 for the same counting rate. Assay of insulin in plasma. Mixtures containing unknown samples are prepared at the same time and in the same way as are standard solutions except that the unknown sample is substituted for the human insulin. Plasma insulin is best determined in...

Chemical And Diagnostic Specificity Of Laboratory Tests

Effect of Hemolysis, Lipemia, Anticoagulants, Medications, Contaminants, and Other Variables WENDELL T. CARAWAY, PH.D. McLaren General Hospital, St. Joseph Hospital, and the Flint Medical Laboratory, Flint, Michigan It is desirable that clinical laboratory procedures have a high degree of diagnostic specificity. Chemical analysis of biologic material is based upon the presence of functional groups in organic compounds or upon the separation and measurement of ions. Such analyses are inherently...

Summary And Conclusion

An immunoassay for plasma insulin in man is presented, based on the reaction of human insulin, competing with beef insulin-I131, with insulin-binding antibodies in the sera of guinea pigs immunized with beef insulin. The method is sensitive to less than 1 U of insulin, permitting measurement of insulin concentrations in 10 to 20 xl of plasma. 2. Human insulin added in vitro to plasma is recovered quantitatively, and measured endogenous insulin concentrations decrease proportionately on...

References

COMMENTARY TO Separation of Tissue and Serum Creatine Kinase Isoenzymes by Ion Exchange Column Chromatography. Clinical Chemistry 20 1 36-40. The measurement of isoenzymes in serum in order to improve the specificity of an enzyme assay for disease detection began with the work of Oscar Bodansky in 1937. He used taurocholate inhibition to differentiate bone, kidney and intestinal alkaline phosphatase ALP, EC 3.1.3.1 in normal serum and in two patients with Paget's disease 1 . Zone...

Caraway Method Of Interpretation

Ation of voltage as a source of error in coulometric titration. Am. J. Clin. Path., 34 195, 1960. 187. Woodard, H. Q. The clinical significance of serum acid phosphatase. Am. J. Med., 27 902-910, 1959. 188. Wootton, I. D. P. Standardization in clinical chemistry. Clin. Chem., 3 401405, 1957. 189. Wr6blewski, F. The clinical significance of transaminase activities of serum. Am. J. Med., 27 911-923, 1959. 190. Wr6blewski, F., and LaDue, J. S. Lactic dehydrogenase activity in blood. Proc. Soc....

Gemini Centrifugal Analyzer

Procedure Clinical Chemistry

Electrode analysis of sodium concentration in whole blood after addition of weighed amounts of NaCl. The actual plasma Na concentration achieved was measured by flame photometry. Fia. 4. Electrode analysis of sodium concentration in whole blood after addition of weighed amounts of NaCl. The actual plasma Na concentration achieved was measured by flame photometry. a small constant. This strongly suggests that some proportion of the sodium in the plasma is inaccessible to the electrode...