Ph

Mobility(i*)-pH curves for ferrohemoglobins in phosphate buffers of 0.1 ionic strength containing dithionite ion. The mobilities recorded in the graph are averages of lb* mobilities in the ascending and descending limbs. concentrated solutions with this dithionite-containing buffer and dialyzing against it under a nitrogen atmosphere. The hemoglobin solutions for the experiments of type 3 were made up similarly, except that they were saturated with carbon monoxide after dilution and...

Leuci Mesensitive Hypoglycemia

Hypoglycemia

Fasting plasma insulin concentrations in various groups of subjects. The subject with plasma insulin concentration greater than 1,500 xU per ml had an islet cell adenocarcinoma with widespread metastases (patient of Dr. J. Field). tion depends on the precise moment of sampling. A very rapid and pronounced fall from the peak concentration would be anticipated in the case of a single secretory spurt. Conversely, a sustained elevation or continued rise in insulin concentration implies a...

Time In Hours

Plasma insulin concentrations during heavy glucose loading experiments in diabetic and nondiabetic subjects. groups but more marked increases were observed in the diabetic subjects. It should be emphasized that insulin-I131 when administered intravenously exhibits a rapid fall in concentration due to a marked and continuous increase in its apparent volume of distribution for a period of about 30 to 60 minutes and to a metabolic turnover rate with a half-time of about 35 minutes (22). It...

Info

Active sites a suggestion in accord with the ideas expressed by Mirsky (42) but not of immunologically active sites on the insulin molecules and 4) the presence of insulin antagonists. The last suggestion has been made many times by previous workers. A joint attack on the problem, utilizing both the specific immunoassay for plasma insulin and an assay method that measures the net biological effect of insulin and its inhibitors would seem to be indicated. The high fasting insulin concentrations...

Contamination

There are many sources of contamination, in the clinical laboratory. Some of these may affect a random specimen and be overlooked or not detected, even with inclusion of standards and controls. Thorough cleaning of glassware is essential. Detergents frequently contain large quantities of sodium and inorganic phosphate, and these will interfere if not completely removed. A simple check to detect residual detergent consists of filling pipets or rinsing glassware with a dilute solution of...

Methods

Guinea pigs were injected subcutaneously at 1 to 4 week intervals with 5 to 10 units of either protamine zinc beef insulin (Squibb) or commercial regular beef insulin (Squibb) emulsified with mannide mono-oleate. Insulin-binding antibodies were detected in all animals after 2 to 3 injections. The antiserum employed in the present study (GP 49, serum 6-25-59) was obtained from a guinea pig immunized with protamine zinc beef insulin without adjuvant and was selected...

Enzymelinked Immunosorbent Assay

QUANTITATIVE ASSAY OF PROTEIN ANTIGEN, IMMUNOGLOBULIN G, BY MEANS OF ENZYME-LABELLED ANTIGEN AND ANTIBODY-COATED TUBES EVA ENGVALL, KARIN JONSSON and PETER PERLMANN From the Department of Immunology, The Wenner-Gren Institute, University of Stockholm, Stockholm (Sweden) Alkaline phosphatase from calf intestinal mucosa has been conjugated to a protein antigen, rabbit IgG. Such conjugates, prepared by glutardialdehyde, have been used in a competitive solid phase immunoassay. In this test...

Immunoassay Of Endogenous Plasma Insulin In Man

Centrations observed in diabetic subjects during the glucose tolerance test are not inconsistent with the less extensive data of Bornstein and Lawrence (1) and Baird and Bornstein (39). Very recently Seltzer and Smith (41), employing the rat diaphragm assay of Vallance-Owen and Hurlock (33), have reported insulin concentrations one hour after glucose, in tolbutamide-sensitive adult diabetics, almost in the normal range, but significantly lower values were observed in juvenile diabetics and...

180 Mg 100 Ml Of Blood Sugar

It is evident that variation in the volumes of solution applied to the paper strips is of no consequence. Generally 100 to 200 fil is applied, the larger volume permitting use of a smaller quantity of tracer beef insulin-I131 for the same counting rate. Assay of insulin in plasma. Mixtures containing unknown samples are prepared at the same time and in the same way as are standard solutions except that the unknown sample is substituted for the human insulin. Plasma insulin is best determined in...

Chemical And Diagnostic Specificity Of Laboratory Tests

Effect of Hemolysis, Lipemia, Anticoagulants, Medications, Contaminants, and Other Variables WENDELL T. CARAWAY, PH.D. McLaren General Hospital, St. Joseph Hospital, and the Flint Medical Laboratory, Flint, Michigan It is desirable that clinical laboratory procedures have a high degree of diagnostic specificity. Chemical analysis of biologic material is based upon the presence of functional groups in organic compounds or upon the separation and measurement of ions. Such analyses are inherently...

Summary And Conclusion

An immunoassay for plasma insulin in man is presented, based on the reaction of human insulin, competing with beef insulin-I131, with insulin-binding antibodies in the sera of guinea pigs immunized with beef insulin. The method is sensitive to less than 1 U of insulin, permitting measurement of insulin concentrations in 10 to 20 xl of plasma. 2. Human insulin added in vitro to plasma is recovered quantitatively, and measured endogenous insulin concentrations decrease proportionately on...

Caraway

Ation of voltage as a source of error in coulometric titration. Am. J. Clin. Path., 34 195, 1960. 187. Woodard, H. Q. The clinical significance of serum acid phosphatase. Am. J. Med., 27 902-910, 1959. 188. Wootton, I. D. P. Standardization in clinical chemistry. Clin. Chem., 3 401405, 1957. 189. Wr6blewski, F. The clinical significance of transaminase activities of serum. Am. J. Med., 27 911-923, 1959. 190. Wr6blewski, F., and LaDue, J. S. Lactic dehydrogenase activity in blood. Proc. Soc....

Gemini Centrifugal Analyzer

Procedure Clinical Chemistry

Electrode analysis of sodium concentration in whole blood after addition of weighed amounts of NaCl. The actual plasma Na concentration achieved was measured by flame photometry. Fia. 4. Electrode analysis of sodium concentration in whole blood after addition of weighed amounts of NaCl. The actual plasma Na concentration achieved was measured by flame photometry. a small constant. This strongly suggests that some proportion of the sodium in the plasma is inaccessible to the electrode...