Demonstration of viral episomes and virion DNA

To demonstrate and distinguish persistent non-integrated episomal slowly migrating or lytical linear higher mobility viral DNA, in situ lysis gel electrophoresis can be used. The method was originally developed by Gardella 64 , and a simplified version is now used modified after ref. 22 . Typical examples for 'Gardella gels' are shown in various reports 11, 22, 36, 64, 65 . The electrophoresis is done in a vertical 1 agarose gel in 1 X TBE 89 mM Tris, 89 mM boric acid, 20 mM EDTA with gel size...