832 Chapter 21 Lipid Biosynthesis mitochondrion, is transferred to the cytosol by the acetyl group shuttle outlined in Figure 21-10.
(a) Write the overall equation for the transfer of one acetyl group from the mitochondrion to the cytosol.
(b) What is the cost of this process in ATPs per acetyl group?
(c) In Chapter 17 we encountered an acyl group shuttle in the transfer of fatty acyl-CoA from the cytosol to the mitochondrion in preparation for ft oxidation (see Fig. 17-6). One result of that shuttle was separation of the mitochondrial and cytosolic pools of CoA. Does the acetyl group shuttle also accomplish this? Explain.
8. Oxygen Requirement for Desaturases The biosynthesis of palmitoleate (see Fig. 21-12), a common unsaturated fatty acid with a cis double bond in the A9 position, uses palmitate as a precursor. Can this be carried out under strictly anaerobic conditions? Explain.
9. Energy Cost of Triacylglycerol Synthesis Use a net equation for the biosynthesis of tripalmitoylglycerol (tri-palmitin) from glycerol and palmitate to show how many ATPs are required per molecule of tripalmitin formed.
When [14C]glucose is added to the balanced diet of adult rats, there is no increase in the total amount of stored triacyl-glycerols, but the triacylglycerols become labeled with 14C. Explain.
Write the sequence of steps and the net reaction for the biosynthesis of phosphatidylcholine by the salvage pathway from oleate, palmitate, dihydroxyacetone phosphate, and choline. Starting from these precursors, what is the cost (in number of ATPs) of the synthesis of phosphatidylcholine by the salvage pathway?
12. Salvage Pathway for Synthesis of Phosphatidylcholine A young rat maintained on a diet deficient in methionine fails to thrive unless choline is included in the diet. Explain.
[14C]acetyl-CoA is added to a rat liver homogenate that is synthesizing cholesterol, where will the 14C label appear in A3-isopentenyl pyrophosphate, the activated form of an isoprene unit?
14. Activated Donors in Lipid Synthesis In the biosynthesis of complex lipids, components are assembled by transfer of the appropriate group from an activated donor. For example, the activated donor of acetyl groups is acetyl-CoA. For each of the following groups, give the form of the activated donor: (a) phosphate; (b) D-glucosyl; (c) phospho-ethanolamine; (d) D-galactosyl; (e) fatty acyl; (f) methyl; (g) the two-carbon group in fatty acid biosynthesis; (h) A3-isopentenyl.
15. Importance of Fats in the Diet When young rats are placed on a totally fat-free diet, they grow poorly, develop a scaly dermatitis, lose hair, and soon die—symptoms that can be prevented if linoleate or plant material is included in the diet. What makes linoleate an essential fatty acid? Why can plant material be substituted?
16. Regulation of Cholesterol Biosynthesis Cholesterol in humans can be obtained from the diet or synthesized de novo. An adult human on a low-cholesterol diet typically synthesizes 600 mg of cholesterol per day in the liver. If the amount of cholesterol in the diet is large, de novo synthesis of cholesterol is drastically reduced. How is this regulation brought about?
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