Immobilized Metal Affinity Chromatography

Immobilised Metal Ion Chromatography

Figure 5 Schematic diagram of immobilized metal ion affinity chromatography. Metal ions are immobilized on the chelating resin, which then binds the target protein reversibly. Elution of the bound protein is carried out with a change in pH, introduction of imidazole in the wash buffer or a metal chelator (e.g., EDTA).

Figure 5 Schematic diagram of immobilized metal ion affinity chromatography. Metal ions are immobilized on the chelating resin, which then binds the target protein reversibly. Elution of the bound protein is carried out with a change in pH, introduction of imidazole in the wash buffer or a metal chelator (e.g., EDTA).

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