Laboratory Manual andCompanies 2003 Workbook in Microbiology 7e

Salmonella, 189t, 190-191, Plate 16, Plate 17 Saprophytes, 110 Satellite test, Plate 34 Scotch tape preparation, 251-254, 252f Sexually transmitted diseases, 213-218, 213t, 214f, 215f Shigella, 189t, 190-191 SIM (sulfide, indole, and motility) medium, 119-120, 120f collection of, 107-108 handling of, 108-109 urine, 207 Spirilla, 33, 34f Spirochetes, 33, 34f, 217-218, Plate 7 Sporothrix schenckii, 248t, 253t Stain(s) acid-fast, 43-45 capsular, 47 for capsules, 50-51 for endospores, 48-50, 49f...

Workbook in Microbiology 7e

Microbiology Mold Slides

And debris, making the fungi more prominent. Slides must be examined with reduced illumination to allow fungal structures to be seen (see colorplate 47). 2. Calcofluor white This reagent is used with most specimen types to detect the presence of fungi by fluorescence microscopy. The cell walls of the fungi bind the stain and fluoresce blue-white or apple green, depending on the filter combination used with the microscope. This stain is useful for examining skin scrapings for the presence of...

Exercise 2 Neisseria and Spirochetes

Microbiology Neisseria

The sexually transmitted diseases are perhaps the most important infections acquired through the urogenital tract, from the social as well as medical points of view. Three frequent infectious diseases of this type are gonorrhea, syphilis, and chlamydial urethritis cervicitis. All three infections are caused by bacteria. Gonorrhea is caused by Neisseria gonorrhoeae syphilis by Treponema pallidum, a spirochete and chlamydial infection by Chlamydia trachomatis. Neisseria gonorrhoeae can be grown...

Experiment 242 Isolation Techniques for Enteric Pathogens

Listeria Isolation Method

Bacterial diseases of the intestinal tract can be highly communicable and may spread in epidemic fashion. Their agents enter the body through the mouth in contaminated food or water, or as a result of direct contacts with infected persons. Among the Enterobacteriaceae, the organisms of pathogenic significance belong to the genera Salmonella, Shigella, and Yersinia. Also certain Escherichia coli strains can produce disease by several mechanisms including invading tissue or producing toxins. Such...

Experiment 201 Isolation and Identification of Staphylococci

The laboratory diagnosis of staphylococcal disease is made by identifying the organism usually S. aureus in a clinical specimen representing the site of infection pus from a skin lesion, sputum when pneumonia is suspected, urine, spinal fluid, or blood . It should be remembered that either S. aureus or S. epidemidis may be harmlessly present on superficial tissues. Special care must be taken not to contaminate the specimen with normal flora, and laboratory results must be interpreted in the...

Providencia Stuartii Characteristics Alpha Hemolysis

Klebsiella Pneumoniae Tsi

Morello-Mizer-Granato I Back Matter I Figures I I The McGraw-Hill Laboratory Manual and Companies, 2003 Workbook in Microbiology, 7 e Plate 1 Staphylococcus aureus in a Gram-stained smear from a colony growing on agar medium left and from the sputum of a patient with staphylococcal pneumonia right . The organisms are gram-positive spheres, primarily in grapelike clusters. The pink cells in the right-hand photo are neutrophils. Plate 2 Streptococcus pyogenes in Gram-stained smears. From a...

Simple Stains

Procedure Microbiological Specimens

As we have seen in Exercise 3, wet mounts of bacterial cultures can be very informative, but they have limitations. Bacteria bounce about in fluid suspensions with Brownian movement or true motility, and are difficult to visualize sharply. We can see their shapes and observe their activity under a cover glass, but it is difficult to form a complete idea of their morphology. An important part of the problem is the minute size of bacteria. Because they are so small and have so little substance,...

Parasitic Helminths

Refractile Objects Cell Culture

Helminths, or worms, are soft-bodied invertebrate animals. Their adult forms range in size from a few millimeters to a meter or more in length, but their immature stages eggs, or ova, and larvae are of microscopic dimensions. Relatively few species of helminths are parasitic for humans, but these few are widely distributed. It has been estimated that 30 of the earth's human inhabitants harbor some species of parasitic worm. There are two major groups of helminths the roundworms, or nematodes,...