Leuci Mesensitive Hypoglycemia

Hypoglycemia

Fasting plasma insulin concentrations in various groups of subjects. The subject with plasma insulin concentration greater than 1,500 xU per ml had an islet cell adenocarcinoma with widespread metastases (patient of Dr. J. Field). tion depends on the precise moment of sampling. A very rapid and pronounced fall from the peak concentration would be anticipated in the case of a single secretory spurt. Conversely, a sustained elevation or continued rise in insulin concentration implies a...

Contamination

There are many sources of contamination, in the clinical laboratory. Some of these may affect a random specimen and be overlooked or not detected, even with inclusion of standards and controls. Thorough cleaning of glassware is essential. Detergents frequently contain large quantities of sodium and inorganic phosphate, and these will interfere if not completely removed. A simple check to detect residual detergent consists of filling pipets or rinsing glassware with a dilute solution of...

Methods

Guinea pigs were injected subcutaneously at 1 to 4 week intervals with 5 to 10 units of either protamine zinc beef insulin (Squibb) or commercial regular beef insulin (Squibb) emulsified with mannide mono-oleate. Insulin-binding antibodies were detected in all animals after 2 to 3 injections. The antiserum employed in the present study (GP 49, serum 6-25-59) was obtained from a guinea pig immunized with protamine zinc beef insulin without adjuvant and was selected...

Enzymelinked Immunosorbent Assay

QUANTITATIVE ASSAY OF PROTEIN ANTIGEN, IMMUNOGLOBULIN G, BY MEANS OF ENZYME-LABELLED ANTIGEN AND ANTIBODY-COATED TUBES EVA ENGVALL, KARIN JONSSON and PETER PERLMANN From the Department of Immunology, The Wenner-Gren Institute, University of Stockholm, Stockholm (Sweden) Alkaline phosphatase from calf intestinal mucosa has been conjugated to a protein antigen, rabbit IgG. Such conjugates, prepared by glutardialdehyde, have been used in a competitive solid phase immunoassay. In this test...

Immunoassay Of Endogenous Plasma Insulin In Man

Centrations observed in diabetic subjects during the glucose tolerance test are not inconsistent with the less extensive data of Bornstein and Lawrence (1) and Baird and Bornstein (39). Very recently Seltzer and Smith (41), employing the rat diaphragm assay of Vallance-Owen and Hurlock (33), have reported insulin concentrations one hour after glucose, in tolbutamide-sensitive adult diabetics, almost in the normal range, but significantly lower values were observed in juvenile diabetics and...

180 Mg 100 Ml Of Blood Sugar

It is evident that variation in the volumes of solution applied to the paper strips is of no consequence. Generally 100 to 200 fil is applied, the larger volume permitting use of a smaller quantity of tracer beef insulin-I131 for the same counting rate. Assay of insulin in plasma. Mixtures containing unknown samples are prepared at the same time and in the same way as are standard solutions except that the unknown sample is substituted for the human insulin. Plasma insulin is best determined in...

Chemical And Diagnostic Specificity Of Laboratory Tests

Effect of Hemolysis, Lipemia, Anticoagulants, Medications, Contaminants, and Other Variables WENDELL T. CARAWAY, PH.D. McLaren General Hospital, St. Joseph Hospital, and the Flint Medical Laboratory, Flint, Michigan It is desirable that clinical laboratory procedures have a high degree of diagnostic specificity. Chemical analysis of biologic material is based upon the presence of functional groups in organic compounds or upon the separation and measurement of ions. Such analyses are inherently...

Info

Po2 Clinical Electrode

Of the urine samples were 1 or more with Benedict's test but were negative with the glucose oxidase test. Two of these urines contained over 0.3 ascorbic acid which was a sufficient quantity to account for the positive test. In addition, among these urines were samples containing galactose, lactose, and fructose. There were 36 urine samples that gave positive reactions with the glucose oxidase test and negative reactions with Benedict's test. In these cases the amount of glucose present was...

Gemini Centrifugal Analyzer

Procedure Clinical Chemistry

Electrode analysis of sodium concentration in whole blood after addition of weighed amounts of NaCl. The actual plasma Na concentration achieved was measured by flame photometry. Fia. 4. Electrode analysis of sodium concentration in whole blood after addition of weighed amounts of NaCl. The actual plasma Na concentration achieved was measured by flame photometry. a small constant. This strongly suggests that some proportion of the sodium in the plasma is inaccessible to the electrode...